The interaction of dialkyl ether lecithins with phospholipase A2 (Naja naja naja).

Chemical Modification of the Histidine Residue in Phospholipase A, (Naja naja naja)

Reaction of phospholipase A, (Naja naja naja) with pbromophenacyl bromide leads to almost complete loss of enzymatic activity. The rate of inactivation is pa-dependent with ,pK, = 6.9 for the ionizing residue. p-Bromophenacyl bromide modifies 0.5 mol of histidinelmol of enzyme as judged by amino acid analysis and incorporation studies with W-labeled reagent. The rate of inactivation is affected...

Zinc and barium inhibit the phospholipase A2 from Naja naja atra by different mechanisms.

The mode of inhibition of the phospholipase A2 (PLA2) enzyme from the Chinese cobra (Naja naja atra) by Zn2+ is qualitatively different from inhibition by Ba2+. Inhibition by Ba2+ shows the kinetic characteristics of a conventional competitive inhibitor acting to displace Ca2+ from a single essential site, but Zn2+ has the paradoxical property of being more inhibitory at high than at low Ca2+ c...

Characterization and physical properties of the major form of phospholipase A2 from cobra venom (Naja naja naja) that has a molecular weight of 11,000.

The major form of phospholipase A2 from cobra venom (Naja naja naja) was prepared in 30% yield and was homogeneous on polyacrylamide gel electrophoresis with and without sodium dodecyl sulfate and on Sephadex G-100 chromatography. The monomer molecular weight is about 11,000 according to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Ultracentrifugation and molecular sieve technique...

Antibacterial activity of an acidic phospholipase A2 (NN-XIb-PLA2) from the venom of Naja naja (Indian cobra)

The resistance of bacteria against the use of conventional antibiotics has become a serious threat to public health and considering the associated side effect with antibiotics; new strategies to find and develop new molecules with novel modes of action has received grate attention in recent years. In this study, when the antibacterial potential of an acidic protein-NN-XIb-PLA2 (Naja naja venom ...

Isolation of phospholipase A isoenzymes from Naja naja venom and their action on membrane-bound enzymes.